Solid Cancer Panel

SOLIDaccuTestTM

SOLIDaccuTestTM is designed to explorer genetic variants associated with solid tumors using NGS technology. It reflected the latest research trends and is optimized for the medical purpose by selecting essential genes for solid cancer diagnosis.

Features
Procedure

NGS workflow for solid cancer diagnosis

Specification
Category Contents
Target Design Target Region / Size DNA : 42 genes (all coding exons) : 120 Kb
RNA : 30 genes (all coding exons) : 90 Kb
Probe type DNA
Probe size About 120mer
Sample Cancer Type Solid cancer
Sample type FFPE tissue, FF tissue
Tumor % ≥ 10%
Input DNA Quality DNA : DIN ≥ 3.0
RNA : DV200 > 50%
Input DNA Quantity DNA : ≥ 200ng
RNA : ≥ 500ng
Sequencing Target capture method  Hybridization (~120mer probes)
Sequencer MiSeqDx, MiSeq
Reagent kit MiSeq® Reagent Kit v3(600 cycles)
Read Cycles 2 x 150 bp (paired-end reads)
Turn Around Time Total 5 days : library prep (3 days) + sequencing (2 days)
Reactions / Kit 20 samples (DNA + RNA)
Sequencing Data  Data throughput DNA : ≥ 200M bp
RNA : ≥ 200M bp
Target coverage Average coverage ≥ 300X . Minimum coverage ≥ 100X
Variants DNA : SNV, INDEL, CNV
RNA : gene-gene fusion, expression level
Analysis Solution NGeneAnalySys™ analysis software
Analysis Time 3hrs / sample
Information
Cat.No Contents Reactions Price
NGB131UIA71 SOLIDaccuTestTM
DNA Library Preparation Kit
96 rxns
NGB131UIA72 SOLIDaccuTestTM
DNA Target Enrichment Kit
8 rxns (up to 96 tests)
NGB132UIA71 SOLIDaccuTestTM
RNA Library Preparation Kit
96 rxns
NGB132UIA72 SOLIDaccuTestTM
RNA Target Enrichment Kit
8 rxns (up to 96 tests)